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KMID : 0613820040140040627
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Journal of Life Science
2004 Volume.14 No. 4 p.627 ~ p.635
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Polymorphism Analysis of the ND-4 Gene for the Origin Determination of Olive Flounder, Paralichthys olivaceus
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Song In-Sun
Jin Deuk-Hee
Choi Suk-Jung
Lee Suk-Keun
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Abstract
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In order to distinguish the genetic polymorphism among the olive flounder (Paralichthys olivaceus) obtained from East sea of Jumunjin, aquaculture of Tongyoung and Geoje, and East sea of North Korea, the ND-4 and cytochrome b genes of olive flounder were divided into 5 regions. Each region was analyzed by degenerating gel electrophoresis scanning (DGES), and by subsequent DNA sequencing. The DGES disclosed characteristic DNA polymorphisms in ND-4-2 and ND-4-3 regions of olive flounder, which were also confirmed by the DNA sequencing. The olive flounders obtained from the different marine areas showed DNA mutations in ND-4-2 region (G390A, C402T, and A411G; GenBank: AB028664), and also showed frequent DNA mutations in ND-4-3 region (C515G, C537T, C538T, A567G, G714A, C736T, G756A, A759T, T817C, and T829G), while the cytochrome b gene showed no DNA mutation both in the DGES and DNA sequencing. These data suggest that the ND-4-2 and ND-4-3 regions are candidate loci to distinguish the origin of olive flounder, and that the DGES used in this study provided fast and reliable informations for the genetic polymorphism.
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KEYWORD
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Paralichthys olivaceus, origin determination, DGES, sequencing
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